Lourdes Georgina IGLESIAS ANDREU,Marco Antonio RAMÍREZ MOSQUEDA
申请号：
MX2017000405
公开号：
MX2017000405A
申请日:
2016.12.15
申请国别(地区):
MX
年份:
2018
代理人:
摘要：
The present disclosure is related to the process followed for in vitro induction and regeneration of friable appearance calluses that also generates Vanilla spp. plants with high percentages of somaclonal variation. These will be used in biotechnological improvement works of this species, selecting the seedlings that present characteristics of interest. This process comprises the following stages: Stage 0. Obtaining the plant material and pre-treatment Stage 1. Induction of the friable calluses Stage 2. Buds regeneration Stage 3. Elongation and rooting Stage 4. Acclimatization and Stage 5. Genetic characterization of the regenerated material. In stage 0, immature capsules with 7 months of maturity are collected in the field. The capsules should be healthy and vigorous. In stage 1, immature seeds are used as explant in semisolid MS medium supplemented with at least 0.5 mg L-1 of thidiazuron (TDZ) cultivating them in darkness. In stage 2, semisolid MS medium supplemented with 2.1 mg L-1 of 6-Benzylaminopurine (BAP) is used. Cultivating them in photoperiods of 16 hours of light/8 hours of darkness. In stage 5, inter-microsatellite sequences ISSR (Inter-Simple Sequence Repeat) are used. With this process plants of Vanilla spp. with a 72.2% polymorphism to each other, are obtained in order to expand the genetic base that in this species is reduced.Esta invención hace referencia al proceso seguido para la inducción y regeneración in vitro callos de apariencia friable que además generen plantas de Vanilla spp. con altos porcentajes de variación somaclonal. Estas serán utilizadas en trabajos de mejoramiento biotecnológico de esta especie, seleccionando las plántulas que presentes características de interés. Este proceso comprende las siguientes etapas: etapa 0. Obtención del material vegetal y pre-tratamiento, etapa 1. Inducción de callo friable, etapa 2. Regeneración de brotes, etapa 3. Elongación y enraizamiento, etapa 4. Aclimatización y etapa 5. Caracterización genética
