A physiological sensing device is provided, which includes a conductive textile layer, an adhesive layer, and a physiological transducer. The adhesive layer is disposed on the conductive textile layer and includes a through hole to expose the conductive textile layer. The physiological transducer is disposed on the adhesive layer, in which the physiological transducer is electrically connected with the conductive textile layer by the through hole. When a user uses the physiological sensing device, the conductive textile layer is in contact with the skin of the user, and the physiological sensing device is bonded with a fabric worn by the user by the adhesive layer.一種生理感測裝置,包括導電織物層、貼附層以及生理傳感器。貼附層設置於導電織物層上,且包括貫孔以露出導電織物層。生理傳感器設置於貼附層上,其中生理傳感器透過貫孔與導電織物層電性連接。當使用者使用生理感測裝置時,導電織物層與使用者的皮膚接觸,且生理感測裝置透過貼附層與使用者穿戴的織物結合。100‧‧‧生理感測裝置112‧‧‧導電織物層114‧‧‧貼附層114A‧‧‧貫孔120‧‧‧生理傳感器130‧‧‧傳感接觸墊