The invention relates to an immunogenic agent comprising a Francisella tularensis protein, F. tularensis lipopolysaccharide (LPS), and glucan particles. The F. tularensis protein may be any variant or fragment thereof, and is preferably chosen from FTT0814, FTT0071, FTT0289, FTT0438, FTT0890, FTT1043 or IgIC. The LPS may be a component thereof, preferably O-antigen. Preferably the glucan particles encapsulate the protein and/or LPS. The protein and LPS may be provided as a fusion. The immunogenic agent preferably elicits an antibody and cellular immune response. The immunogenic agent may be formulated as a pharmaceutical composition and may be used in the treatment or prevention of tularemia infection. Methods of producing the immongenic composition or pharmaceutical composition are also disclosed. The F. tularensis proteins FTT0814, FTT0071, FTT0289, FTT0438, FTT0890, FTT1043 or IgIC may be used in the treatment or prevention of tularemia infection.
