An injectable tissue engineered cartilage in vitro construction apparatus, comprising an incubator (1), a centrifugal device arranged in the incubator (1), and a control system for controlling a temperature in the incubator (1) and an action of the centrifugal device. The centrifugal device comprises a centrifuge, a container base (2) mounted onto the centrifuge, a dosing system for adding preparation reagent, and a stirring system for stirring the reagent. With a controller (17) and a pH sensor (16), a measured pH value will be transmitted by the pH sensor (16) to the controller (17) in real time. The controller (17) controls an action of a driving mechanism via a control module II (21), to drive a piston rod to add NaOH solution into a container tank (6). When the pH value of the solution falls in a predetermined range, the driving mechanism is stopped, to precisely control the pH value of the solution. With the stirring system and the centrifugal device, the solution can be stirred and centrifuged as desired, to eventually in vitro construct an injectable tissue engineered cartilage. This relief the burden of the medical staff and improve production efficiency.