DEVELOP CENTER FOR BIOTECHNOLOGY;NATIONAL HEALTH RESEARCH INSTITUTES
发明人:
TSAI, SHIH CHONG,蔡士昌,YUAN, TA TUNG,阮大同,TSENG, SHIH CHONG,曾世祺,LAI, JIANN SHIUN,赖建勳,賴建勳,WU, CHIA CHENG,吴佳城,吳佳城,HUANG, CHAO YANG,黄朝阳,黃朝暘,TSAI, YA WEI,蔡亚薇,蔡亞薇,LOK, YING YUNG,骆映融,駱映融,WU, CHUNG
申请号：
TW104144520
公开号：
TWI579304B
申请日:
2015.12.30
申请国别(地区):
TW
年份:
2017
代理人:
摘要：
The present invention relates to a humanized antibody, or a binding fragment thereof, wherein the humanized antibody binds human ENO1 (GenBank: AAH50642.1), wherein the antibody comprises a light chain variable region (VL) domain comprising a CDR1 having the amino acid sequence LCDR1 (RASENIYSYLT； SEQ ID NO: 6) and a CDR2 having the amino acid sequence LCDR2 (NAKTLPE； SEQ ID NO: 7) and a CDR3 having the amino acid sequence LCDR3 (QHHYGTPYT； SEQ ID NO: 8) and an antibody heavy chain variable region (VH) domain comprising a CDR1 having the amino acid sequence HCDR1 (GYTFTSCVMN； SEQ ID NO: 3), a CDR2 having the amino acid sequence HCDR2 (YINPYNDGTKYNEKFKG； SEQ ID NO: 4) and a CDR3 having the amino acid sequence HCDR3 (EGFYYGNFDN； SEQ ID NO: 5), wherein framework regions in the light chain variable region (VL) domain and the heavy chain variable region (VH) domain comprise amino acid sequences from a human immunoglobulin.本發明係關於一種可與人類ENO1蛋白(GenBank：AAH50642.1)抗-人類α-烯醇化酶(ENO1)結合的人源化抗體或其結合片段，該抗體包含一其包含具有胺基酸序列LCDR1(RASENIYSYLT；SEQ ID NO：6)之CDR1、具有胺基酸序列LCDR2(NAKTLPE；SEQ ID NO：7)之CDR2與具有胺基酸序列LCDR3(QHHYGTPYT；SEQ ID NO：8)之CDR3的輕鏈可變區，及一其包含具有胺基酸序列HCDR1(GYTFTSCVMN；SEQ ID NO：3)之CDR1、具有胺基酸序列HCDR2(YINPYNDGTKYNEKFKG；SEQ ID NO：4)之CDR2與具有胺基酸序列HCDR3(EGFYYGNFDN；SEQ ID NO：5)之CDR3的重鏈可變區，其中位於該輕鏈可變區(VL)及重鏈可變區(VH)中之骨架區(framework region)包含源自人類免疫球蛋白之胺基酸序列。
