MILIEU DE CULTURE POUR BACTÉRIES DU GENRE CLOSTRIDIUM EXEMPT DE CONSTITUANTS D'ORIGINE ANIMAL ET PROCÉDÉ DE PRODUCTION D'UN SURNAGEANT CONTENANT UNE OU PLUSIEURS PROTÉASES À ACTIVITÉ COLLAGÉNOLYTIQUE ET GÉLATINOLYTIQUE
The present invention refers to a culture medium and a process for producing proteases with collagenolytic and gelatinolytic activity by bacteria of the genus Clostridium. Particularly, the present invention refers to an animal product-free culture medium for C. histolyticum, characterized by comprising non-animal origin peptones, preferably vegetable peptones, yeast extract and the amino acids cysteine and arginine. The present invention also refers to a process for producing Clostridium histolyticum liquid culture supernatant comprising one or more proteases with collagenolytic and gelatinolytic activity, and pharmaceutical composition comprising as active ingredient the supernatant or the supernatant optionality purified.
