The present invention relates to a hybrid promoter, in which a whole or a part of a CMV enhancer, a whole or a part of a β-actin promoter, a whole or a part of a CMV promoter, and a whole or a part of a β-actin intron are operably linked to each other, a recombinant vector comprising the same, a transformant transformed with the recombinant vector, a pharmaceutical composition comprising the recombinant vector or the transformant, and a method for preparing a target protein using the recombinant vector or the transformant. The hybrid promoter of the present invention is able to induce high expression of a target protein in a eukaryotic cell. Therefore, the hybrid promoter of the present invention can be effectively used for the development of an antibody or the production of a DNA vaccine
