The invention relates to combined multistage microbial preparations that are effective for enhancement of the biological skin barrier and maintenance of healthy skin microbiota. The preparations might be used as cosmetic or medicinal products. Combined multistage microbial cosmetic product are intended for an invigoration of the natural biological reinforcement barrier on the skin and mucosal surfaces weakened in case of chronic problems associated with dysbiosis such as atopic dermatitis, acne, rosacea and vitiligo or acute problems caused by damage of the skin such as burns, cuts and contusions. The preparations comprises a set of compositions for sequential applications of compounds able (1) to dissolve biofilms formed by the pathogenic microorganisms in the skin and suppress the viability of the microorganisms released from the biofilms in the first stage, (2) to calm an inflammation caused by the imbalanced immune system and restore the efficient biological barrier in the second stage, (3) to achieve the restoration of normal physiological microflora disturbed by the dysbiosis in the third stage, and (4) to contribute to the long term nonspecific skin defense and normal consistency and nutrition in the skin. The beneficial substances may be applied onto the skin in the form of oil emulsions, creams, ointments, gels, and other cosmetic or medical formulations.
