Louis Casteilla,Valérie Planat-Benard,Luc Penicaud,Carine Chanut
申请号：
US12161975
公开号：
US08945920B2
申请日:
2007.01.26
申请国别(地区):
US
年份:
2015
代理人:
摘要：
The invention concerns a method for culturing cells derived from the adipose tissue and in particular the stromal vascular fraction (SVF) to induce formation of cardiomyocytes, the use of the cells obtained by said culture method to reconstitute an ischemized cardiac zone, in particular following an infarction, as well as a pharmaceutical composition containing said cells. The method for obtaining cardiac cells comprises at least the following steps: a) selecting cardiomyogenic cells from the stromal vascular fraction (SVF) b) culturing the cells selected at step a) in a liquid medium optimized for expanding ex vivo the cardiomyogenic cells c) maintaining and expanding said cells by successive passes in the liquid medium and d) obtaining cardiac cells.
