Alexander Deiters,James B. Hemphill,Aravind Asokan,Erin Borchardt
申请号：
US15559898
公开号：
US20180073002A1
申请日:
2016.04.08
申请国别(地区):
US
年份:
2018
代理人:
摘要：
Disclosed herein is a genetically encoded light- or chemically-activated Cas9 engineered through the site-specific installation of an activatable lysine amino acid. Such activatable Cas9 proteins can be used in CRISPR/Cas9 systems to control gene expression temporally, spatially, or both. Systems, methods, kits, and compositions for manipulation of sequences and/or activities of target sequences are provided. Also provided are methods of directing CRISPR complex formation in cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas9 system.
