Josef Burg,Bernhard Hilger,Thorsten Kaiser,Wolfgang Kuhne,Lars Stiens,Claus Wallerius,Frank Zettl
申请号：
US13386175
公开号：
US20120177640A1
申请日:
2010.07.22
申请国别(地区):
US
年份:
2012
代理人:
摘要：
A general method is provided for the production of purified antibodies by separation of an antibody molecule from an antibody variant by chromatographic methods, e.g. to enhance therapeutic efficacy, by for example choosing a specific harvesting time point and/or a specific purification scheme. The current invention thus reports a method for producing an antibody composition comprising an antibody molecule and a variant thereof, comprising the following steps: providing a sample comprising the antibody molecule and a variant thereof, determining the presence of the antibody molecule and/or a variant thereof and/or the ratio of the amount of the antibody molecule or variant thereof to the sum of the amounts of the antibody molecule and the variant thereof, in an aliquot of said sample, determining a subsequent harvesting time point and/or antibody purification scheme on basis of the data obtained before, thereby producing an antibody composition comprising the antibody molecule and a variant thereof.
