A method for producing a mesenchymal stem cell (MSC) that secretes neurotrophic factor (NTF), wherein a population of undifferentiated mesenchymal stem cells (MSC) is derived from basic fibroblast growth factor (bFGF), platelet-derived Incubating in a differentiation medium comprising growth factor (PDGF), heregulin and cAMP. [Selection] Figure 25神経栄養因子(NTF)を分泌する間葉系幹細胞(MSC)を作製する方法であって、未分化間葉系幹細胞(MSC)の集団を、塩基性線維芽細胞増殖因子(bFGF)、血小板由来増殖因子(PDGF)、ヘレグリンおよびcAMPを含む分化用培地においてインキュベーションすることを含む方法。【選択図】図25