本发明公开一种B7H4/1E10单克隆抗体及其应用,该抗体的轻链可变区的3个互补决定区序列分别为:Lys Ser Val Ser Thr Ser Gly Tyr Ser Tyr;Leu Val Ser;Gln His Ile Arg Glu Leu Thr Arg;重链可变区的3个互补决定区序列分别为:Gly Phe Ser Leu Thr Ser Tyr Gly;Ile Trp Ala Gly Gly Ser Thr;Ala Arg Glu Arg Ala Arg Leu Leu Arg Ile Asn Ala Met Asp Tyr。该单克隆抗体效价可达1×10‑6,亲和力高。