An animal restraint device for restraining an animal, such as a rodent, for conducting experiments and collecting data. Restraining devices include a cranial restraint to secure the head and torso of an animal, and a caudal restraint configured to secure the torso of an animal. Restraining devices are made of a flexible material and one or more adjustable fasteners. Animal restraining systems include the animal restraint device and a platform for holding an animal restrained in the restraint device. Methods of using the restraining devices and systems are also provided.
