The present invention, which provides mixing and the transgenosis of recombination phagemid vector expression, has carrier in target cell transduction. Recombination phase granule include at least one transgene expression box which encode a kind of medicament, play biological effect Target cell, it is characterised in that phase granule includes its genome for lacking its at least one 50% bacteriophage of genome. The present invention, which expands to, uses such phasmid expression system as research tool, and delivering transgenosis is in various gene therapies application, DNA and/or peptide vaccine delivering and imaging technique. The present invention expands to external, internal or manufacture in situ method viral vector, such as recombination gland cancer relevant viral (rAAV) or slow virus carrier (rLV), for the genetic constructs used in this approach.