The present invention is directed to a cell sheet for transplantation, having mesenchymal stem cells at an average cell density of 3.0 x 104cells/cm2or less on the surface thereof. The present invention is also directed to a method for producing a cell sheet for transplantation, including a step of seeding 3.0 x 105cells/cm2or less of mesenchymal stem cells onto a cell culture carrier, wherein the carrier has a three-dimensional structure consisting of fibers, and a step of culturing the mesenchymal stem cells, thereby preparing a cell sheet having mesenchymal stem cells at an average cell density of 3.0 x 104cells/cm2or less.本發明係關於一種生物體移植用細胞片，其於其表面具有平均細胞密度為3.0×104細胞/cm2以下之間葉系幹細胞。又，本發明係關於一種生物體移植用細胞片之製造方法，其包括以下之步驟：於具有包含纖維之三維構造之細胞培養載體上，以3.0×105細胞/cm2以下之細胞數播種間葉系幹細胞；及培養間葉系幹細胞而製備平均細胞密度為3.0×104細胞/cm2以下之細胞片。
