The present invention provides a digestive tract multi-region liquid biopsy sampling device. The digestive tract multi-region liquid biopsy sampling device includes an enclosure, and a plurality of sampling chambers formed in the enclosure. The enclosure includes through holes cut in the wall of the enclosure corresponding to each sampling chamber, and targeted dissolution membranes covering the through holes on different sampling chambers that can dissolve at different targeted regions. Expandable materials that can absorb liquids, arranged in the sampling chambers at the positions of the expandable materials corresponding to the through holes, therefore the expandable materials expand after absorbing liquid and close the through holes.
