Methods of tissue manipulation are provided. Aspects of the methods include non-surgical methods for manipulating one or more tissues, such as human tissues. In some versions, the tissues are subcutaneous and the methods include differentiating at least a first tissue and a second tissue. Embodiments of the methods include inducing relative motion between a first tissue and a second tissue by, for example, applying pressure to one or more of the tissues and/or by inducing movement of at least a portion of a subjects body which is operatively connected to a tissue. The disclosed subject matter finds use in a variety of different applications, including alleviating symptoms of overuse conditions.
