FALKENSTEIN ROBERTO,KOEHNLEIN WOLFGANG,KUHNE WOLFGANG
申请号：
IN1891/CHENP/2013
公开号：
IN2013CN01891A
申请日:
2013.03.08
申请国别(地区):
IN
年份:
2014
代理人:
摘要：
Herein is reported a method for the purification of a protein comprising erythropoietin and a single poly (ethylene glycol) residue from reaction by products or not reacted starting material by a cation exchange chromatography method. It has been found that by employing a cation exchange SP Sephacryl S 500 HR chromatography material conditioned to a conductivity of 21 m S/cm and a linear gradient elution a fusion protein of erythropoietin and a single poly (ethylene glycol) residue can be obtained in a single step with high purity and yield.
