Patrick Maria Franciscus Derkx;Bo Spange Sørensen;Karsten Matthias Kragh;Anja Kellet-Smith Hemmingen;Rie Mejldal
发明人:
Patrick Maria Franciscus Derkx,Anja Kellet-Smith Hemmingen,Rie Mejldal,Bo Spange Sørensen,Karsten Matthias Kragh
申请号：
US12339718
公开号：
US08178336B2
申请日:
2008.12.19
申请国别(地区):
US
年份:
2012
代理人:
摘要：
We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 307 to lysine (K) or arginine (R), with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Preferably, the PS4 variant polypeptide further comprises an amino acid substitution at position 70, preferably G70D. The amino acid at positions 272 and 303 of the sequence of the are preferably histidine (H) and glycine (G).
