This invention generally relates to a composition and method for inducing the expansion and/or regeneration of CD34-positive adult stem cell population, which is useful for developing drugs/vaccines and/or therapies against a variety of ageing-related degenerative diseases in humans. Also, the present invention teaches the production and purification methods required for making high quality and high quantity of small hairpin-like RNA (shRNA) compositions, such as microRNA precursors (pri-miRNA and pre-miRNA) and short interfering RNAs (siRNA), which are useful for treating human ageing-related diseases, such as, but not limited, Alzheimer’s diseases, Parkinson’s diseases, osteoporosis, diabetes, and cancers. The resulting shRNAs, preferably pre-miRNAs, are useful for developing therapeutic drugs against human degenerative diseases, particularly through a mechanism to induce CD34-positive stem cell expansion and/or regeneration.本發明大體上係關於一種用於誘導CD34陽性成體幹細胞群體之增殖及/或再生之組合物及方法,其可用於開發針對人類之多種衰老相關退化性疾病的藥物/疫苗及/或療法。此外,本發明教示製造高品質且大量的小髮夾型RNA(shRNA)組合物所需之製造及純化方法,該等組合物諸如microRNA前驅物(pri-miRNA及pre-miRNA)及短干擾RNAs(siRNA),其可用於治療人類衰老相關疾病,諸如但不限於阿茲海默症(Alzheimers disease)、帕金森氏症(Parkinsons disease)、骨質疏鬆症、糖尿病及癌症。所得shRNA、較佳pre-miRNAs可用於開發針對人類退化性疾病之治療藥物,特定言之經由誘導CD34陽性幹細胞增殖及/或再生之機制進行。
