A method for acquiring a fluorescent image including a step of irradiating a sample with excitation light from a position distanced from the sample to generate fluorescence, and detecting the generated fluorescence by a two-dimensional detector to obtain a fluorescent image at least one step of irradiating the sample with excitation light in the same manner in the condition that entry of fluorescence from a part emitting the strongest fluorescence at the time when fluorescence is to be detected is substantially blocked, to obtain a fluorescent image by the two-dimensional detector a step of integrating at least two fluorescent images including the fluorescent image obtained in the step and at least one fluorescent image obtained in the step into one image and a step of displaying the image integrated in the step.
