Relates to a separation method and a cell line derived from the cambium of herbaceous plant having a storage root, and characterized in that it is obtained without dedifferentiation process another forming layer containing storage root tissue of herbaceous plant having a storage root and more about the separation method and cambium-derived cell line with a uniform division potential to be. Cell line derived from the cambium of herbaceous plant having a storage root according to the present invention has a high ability to divide, as a homogeneous cell line, not through the de-differentiation process, it is stable during culture. Therefore, as will be grown in large quantities within a short time the cell lines by optimizing the growth. Therefore, the cell line derived from the cambium of herbaceous plant having a storage root according to the present invention is to be able to mass produce useful plant culture that are difficult to open field cultivation period, the selection of cultivated land, by various problems such as the cultivation costs.貯蔵根を有する草本植物の形成層由来細胞株及びその分離方法に関し、より詳細には貯蔵根を有する草本植物の形成層含有貯蔵根組織で別の脱分化過程なしに取得されることを特徴とする分裂能を有する均一な形成層由来細胞株及びその分離方法に関する。本発明による貯蔵根を有する草本植物の形成層由来細胞株は高い分裂能を有し、均一な細胞株として、脱分化過程を経ずに、培養時に安定的である。そのため、この増殖を最適化することによって細胞株を短い時間内に大量に増殖させられるようになる。従って、本発明による貯蔵根を有する草本植物の形成層由来細胞株は耕作期間、耕作地の選定、耕作費などの様々問題によって露地では培養が困難な有用植物体を大量生産できるようにする。