蠕虫卵、特に鞭虫卵の生物学的活性を測定する方法
- 专利权人:
- 发明人:
- 申请号:
- JP20110509973
- 公开号:
- JP5753778(B2)
- 申请日:
- 2009.05.20
- 申请国别(地区):
- 日本
- 年份:
- 2015
- 代理人:
- 摘要:
Determining the biological activity of helminth eggs, comprises: (a) determining the stages of embryonic development of helminth eggs; (b) determining the metabolic activity of embryonated helminth eggs; (c) determining the inducibility of the gene expression in embryonated helminth eggs; (d) microscopically determining the motility of helminth larva present in the egg, and/or determining the hatching from helminth larva in the subject, where the intact embryonated helminth eggs recovered from intestinal contents are quantified in comparison to an internal standard. Determining the biological activity of helminth eggs, comprises: (a) determining the stages of embryonic development of helminth eggs using quantitative PCR-analysis and marker sequences for determining the copy number of the genomic DNA; (b) determining the metabolic activity of embryonated helminth eggs using biochemical and/or molecular biological methods; (c) determining the inducibility of the gene expression in embryonated helminth eggs;
- 来源网站:
- 中国工程科技知识中心