Van Den Brink, Johannes Maarten,Lund, Martin,Jacobsen, Jonas,Hansen, Sari Charlotte,Jeppesen, Iben
申请号：
NZ74274513
公开号：
NZ742745A
申请日:
2013.05.22
申请国别(地区):
NZ
年份:
2019
代理人:
摘要：
The present invention relates to a method for making an isolated chymosin polypeptide variant comprising the steps: (a): making an alteration in a parent polypeptide having chymosin activity, wherein the alteration comprises a substitution in an amino acid position corresponding to position 117； and (b): producing and isolating the altered polypeptide of step (a) and thereby obtaining the isolated chymosin polypeptide variant, wherein the variant has chymosin activity； and wherein: (i): the amino acid position of the parent polypeptide is determined by an alignment of the parent polypeptide with the polypeptide of SEQ ID NO: 1 (bovine chymosin) – i.e. the polypeptide of SEQ ID NO: 1 is used to determine the corresponding amino acid sequence in the parent polypeptide； and (ii): the parent polypeptide has at least 95% sequence identity with the mature polypeptide of SEQ ID NO: 2 (camel chymosin), which is from amino acid position 59 to amino acid position 381 of SEQ ID NO: 2； and wherein the isolated chymosin polypeptide variant has a chymosin activity giving a higher C/P ratio as compared to the C/P ratio of camel chymosin comprising the mature polypeptide of SEQ ID NO: 2； and wherein said method is not performed within a human body. The present invention also relates to chymosin variants produced by such methods, and to methods for making foods or feed products comprising adding an effective amount of the isolated chymosin polypeptide variants.
