METHOD OF DIFFERENTIATION OF O1 VIBRIO CHOLERAE STRAINS OF EL TOR BIOTYPE WITH VARIOUS EPIDEMIOLOGICAL SIGNIFICANCE BY METHOD OF MULTIPLEX POLYMERASE CHAIN REACTION
Federalnoe kazennoe uchrezhdenie zdravookhraneniya "Rossijskij nauchno-issledovatelskij protivochumnyj institut "Mikrob" Federalnoj sluzhby po nadzoru v sfere zashchity prav potrebitelej i blagopoluchiya cheloveka ("RosNIPCHI "Mikrob")
发明人:
Agafonova Elena Yurevna,Agafonov Dmitrij Alekseevich,Smirnova Nina Ivanovna
申请号：
RU20180100692
公开号：
RU2671412(C1)
申请日:
2018.01.10
申请国别(地区):
俄罗斯
年份:
2018
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention relates to the field of medical microbiology and is intended for the differentiation of strains of V. cholerae of El Tor biotype. PCR is performed in one step in two reaction mixtures, each containing primers to the genes vc0180, vc0514, ctxA and tcpA. Toxigenic epidemic strains of ctxAtcpAVSPare characterized by the formation of amplified fragments corresponding to genes vc0180, vc0514, ctxA and tcpA. Nontoxigenic ctxAtcpAVSPstrains with a potential epidemic danger are characterized by the presence of amplicons corresponding to genes vc0180, vc0514 and tcpA. Nontoxigenic epidemic-safe strains of ctxAtcpAVSPor ctxAtcpAVSPare characterized by the presence of an amplicon corresponding to the gene tcpA, or the absence of all amplicons.EFFECT: invention provides the possibility of differentiating strains of strains of Vibrio cholerae of El Tor biotype to toxigenic epidemically dangerous, non-toxic, potentially epidemically dangerous and non-toxic epidemically safe.1 cl, 1 dwg,
