STROP, PAVEL,DORYWALSKA, MAGDALENA GRAZYNA,RAJPAL, ARVIND,SHELTON, DAVID,LIU, SHU-HUI,PONS, JAUME,DUSHIN, RUSSELL
申请号：
CA2813411
公开号：
CA2813411C
申请日:
2011.11.03
申请国别(地区):
CA
年份:
2016
代理人:
摘要：
The present invention provides engineered polypeptide conjugates (e.g., antibody- drug-conjugates, toxin-(biocompatible polymer) conjugates, antibody-(biocompatible polymer) conjugates, and bispecific antibodies) comprising acyl donor glutamine-containing tags and amine donor agents. In one aspect, the invention provides an engineered Fc-containing polypeptide conjugate comprising the formula (Fc-containing polypeptide)-T-A, wherein T is an acyl donor glutamine-containing tag engineered at a specific site or comprises an endogenous glutamine made reactive by the Fc-containing polypeptide engineering, wherein A is an amine donor agent, and wherein the amine donor agent is site-specifically conjugated to the acyl donor glutamine-containing tag or the endogenous glutamine. The invention also provides methods of making engineered polypeptide conjugates using transglutaminase.
