The invention relates to a device for separation of fluidal biological material components, particularly separation of components of the material collected by liposuction for subsequent transfer of the adipose tissue in an injection form. The device comprises a container with an outlet duct arranged in it, the outlet duct having its upper end adapted for collecting the material, and its lower end that is in fluid communication with the inner space of the container at its bottom. Further, the invention relates to a separating float for separation of the fluidal biological material components, the float being intended to be used in the device, wherein the float comprises at least one by-pass opening for passage of the fluidal biological material contained in the container from the area below the separating float into the area above it. The invention also relates to a kit including the device and the float.