DEPARTMENT OF BIOTECHNOLOGY;ALL INDIA INSTITUTE OF MEDICAL SCIENCE
发明人:
SARMAN SINGH,AYAN DEY
申请号：
BRPI0904621
公开号：
BRPI0904621A2
申请日:
2009.02.10
申请国别(地区):
BR
年份:
2015
代理人:
摘要：
A novel recombinant chimera of DNA construct having esat-6 region of Mycobacterium tuberculosis and kinesin region of Leishmania donovani cloned together on two sides of self cleaving peptide in a DNA vaccine vector pVAX-1 wherein the chimeric construct is operatively linked to a transcriptional promoter thus capable of self replication and expression within the mammalian cell, and the process of preparation thereof comprising: analysis of the predicted protein sequence of kinesin motor domain and esat-6 domain using Promiscuous MHC Class-1 Binding Peptide Prediction Servers； amplification of gene coding for kinesin motor domain and esat-6 domain； cloning of kinesin esat-6 gene region in pGEM-T&trade； vector for sequence analysis； generation of chimeric construct by directional cloning in pVAX-1 vector. In-vitro expression analysis of kinesin motor domain and esat-6 domain from the clones using cell free translation system and immunogenicity studies； and splenocyte proliferation and cytokines studies using the above mentioned constructs.
