Provided is a production method of composition with fatigue soothing effect, which includes: providing a silk glue, wherein the silk glue is made by cocoon shell and water； diluting the silk glue to obtain a diluted silk glue； mixing a plurality of raw materials to form a precursor mixture, wherein the raw materials consist of: 1 to 20 parts of diluted silk glue, 3 to 20 parts of guarana extract, 0.5 to 10 parts of [alpha]-aminobutyric acid, 0.1 to 2 parts of tea extract, 0.1 to 2 parts of taurine and 580 to 977.8 parts of water； heating the precursor mixture to the temperature of 90 ~ 100 DEG C so as to obtain a heated precursor mixture； adding a flavoring agent to the heated precursor mixture to form a mixture, wherein the flavoring agent includes 10 to 100 parts of juice； and cooling the mixture to the room temperature so as to obtain a composition with fatigue soothing effect.一種具有舒緩疲勞效果的組成物的製造方法，包括提供一蠶絲膠水，其中該蠶絲膠水係由繭殼與水製成；稀釋該蠶絲膠水以取得一經稀釋的蠶絲膠水；混合複數原料以形成一前驅混合物，其中該些原料包括：1~20份的經稀釋的蠶絲膠水、3~20份的瓜拉納萃取物、0.5~10份的γ-氨基丁酸、0.1~2份的茶葉萃取物、0.1~2份的牛磺酸以及580~977.8份的水；加熱該前驅混合物至90~100℃以取得一經加熱的前驅混合物；添加一調味劑至該經加熱的前驅混合物而形成一混合物，其中該調味劑包括10~100份的果汁；以及冷卻該混合物至室溫而取得該具有舒緩疲勞效果的組成物。
