A device (1) for collecting and transferring samples of biological material, where the device comprises at least: a support body (2) having at least a first end portion (2a) and at least a gripping portion (2b), and a plurality of fibers (6) attached to and arranged on the first portion (2a) of the support body (2) by flocking, such as to define a flocked collecting portion (3) suitable for collecting, on the collecting portion (3), a quantity of a sample of biological material, where the support body (2) is made of polyamide (PA or nylon), in particular polyamide 66 (PA66 or nylon 66). The support body can be provided with at least a weakened portion (4) suitable for enabling a selective breaking of the support body and a separation of the collecting portion (3) at least from a part of the gripping portion (2b).