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MICROVÉSICULE ET SON PROCÉDÉ DE PRODUCTION
专利权人:
KATSURA, MISAKO;LI, ZHONG; ZHONG;LI
发明人:
FENG, LUO
申请号:
EP14764391
公开号:
EP2975125A4
申请日:
2014.03.13
申请国别(地区):
EP
年份:
2016
代理人:
摘要:
The present invention provides a method for producing microvesicles comprising a transgene product and/or a lentiviral RNA comprising a transgene, comprising the steps of: culturing a cell into which the transgene has been introduced using a lentiviral vector in vitro to extracellularly release microvesicles comprising the transgene product and/or the lentiviral RNA comprising the transgene, wherein said lentiviral vector is deficient in at least one structural protein gene and comprises the transgene under control of a telomerase reverse transcriptase (TERT) gene promoter in a lentiviral genome sequence, and collecting the microvesicles released; and a microvesicle obtained accoding to this method and its use.
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