The method involves measurement of impedance for human body region at high frequency (Z HF ) and low frequency (Z LF ) by means of electrodes fastened to a human body. An estimate for the volume of fluid contained in the tissues of a human body region is derived based on Z HF value. An estimate for the volume of extracellular fluid contained in the tissues of a human body region is derived based on Z LF value. The increment of metabolic component of the volume of extracellular fluid (with the said metabolic component being connected with synthesis and utilization of energy carriers in the human body) is determined by calculating the increment of the volume of all fluid as compared to the value obtained from the previous measurement, calculating the increment of the volume of extracellular fluid as compared to the value obtained from the previous measurement, and subsequent calculation of difference between the said increment of the volume of all fluid and increment of the volume of extracellular fluid. A value of increment of glucose concentration is derived via normalization of the said increment of metabolic component of extracellular fluid volume. Glucose concentration in human blood G(t k ) is determined by summing up the said value of glucose concentration increment and value of glucose concentration in blood derived at the previous stage of measurements. The method allows determining glucose concentration in human blood non-invasively and with high accuracy.
