The present disclosure provides for systems and method for producing hybrid seed. In various embodiments, the disclosure provides a system for the high-throughput, nondestructive sampling of seeds. In another embodiment, a high-throughput, nondestructive method for producing hybrid seeds comprises removing a sample from a plurality of seeds in the population while preserving the germination viability of the seed and analyzing the sample for the presence or absence of one or more genetic markers indicative of a male-sterile gene.
