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METHOD FOR TREATING CANCER BASED ON THE USE OF EXOSOM
专利权人:
АйТиЭйч ИММЬЮН ТЕРАПИ ХОЛДИНГЗ АБ (SE)
发明人:
ГАБРИЭЛЬССОН Сусанне (SE)
申请号:
RU2012103482/15
公开号:
RU2012103482A
申请日:
2010.07.02
申请国别(地区):
RU
年份:
2013
代理人:
摘要:
1. A method for producing specific immunomodulatory exosomes, comprising the following steps: (i) transforming B cells by infection of said B cells with Epstein-Barr virus to a latent stage, achieving expression of a gp350 ligand capable of binding to the CD21 receptor of a native B cell; (ii ) culturing the transformed B cells obtained in step (i); (iii) collecting exosomes isolated from the transformed B cells cultured in step (ii), which include one or more parts capable of binding to a native B cell, and loaded directly and / or indirectly with one or more antigens and / or immunosuppressants, while one or more antigens are selected, for example, from one or more cancer antigens, one or more viral antigens, one or more bacterial antigens, one or more immunosuppressors or any combination thereof. 2. The method according to claim 1, which further optionally comprises neutralizing latent membrane protein 1 (LMP-1) on exosomes. The method according to claim 2, wherein LMP-1 is neutralized using a Fab fragment molecule. The method according to claim 1, wherein one or more cancer antigens, one or more antigens expressed on the surface of the tumor cell, or an antigen-active fragment thereof, a fragment of a tumor antigen peptide containing from 8 to 12 amino acid residues or from 15 to 24 amino acid residues, have the ability to stimulate T cells, tumor cell lysate, or mixtures thereof. 5. The method according to any one of the preceding paragraphs, in which the exosomes are loaded indirectly by culturing the transformed B cells in the presence1. Способ получения специфических иммуномодулирующих экзосом, включающий следующие стадии:(i) трансформация В-клеток путем инфицирования указанных В-клеток вирусом Эпштейна-Барра в латентную стадию с достижением экспрессии лиганда gp350, способного связываться с рецептором CD21 нативной В-клетки;(ii) культивирование трансформированных В-клеток, полученных на стадии (i);(iii) сбор экзосом, выделяемых из трансформированных В-клето
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