GREG HERMANSON,PETER CZERNEY,SURBHI DESAI,MATTHIAS S. WENZEL,BOGUSLAWA DWORECKI,FRANK G LEHMANN,CHRISTINE MARIE NLEND
申请号：
GB201214580
公开号：
GB2493843A
申请日:
2012.08.15
申请国别(地区):
GB
年份:
2013
代理人:
摘要：
Dihydrobenzo[e]indol-(2E)-ylidene compounds of formulae IIa, IIb, IIIa and IIIb: wherein R1, R2, R5, R6 are aliphatic, heteroaliphatic, sulfoalkyl, heteroaliphatic with terminal SO3, a PEG group P-Z where P is an ethylene glycol, diethylene glycol or polyethylene glycol group, a sulfonamide group L-SO2NH-P-Z or a carboxamide group L-CONH-P-Z Z is H, CH3, alkyl or heteroalkyl R7, R8, R11, R12, R13and R14 are H or substituents X is -OH, -SH, -NH2, -NH-NH2, -F, -Cl, -Br, -I, -NHS (N-hydroxysuccinimidyl/sulfosuccinimidyl), -O-TFP (2,3,5,6-tetrafluorophenoxy), -O-STP (4-sulfo-2,3,5,6-tetrafluorophenoxy), -O-benzotriazole, benzotriazole, -NR-L-O-phosphoramidite, -NR-L-SH, -NR-L-NH-NH2, -NR-L-CO2H, -NR-L-CO-NHS , -NR-L-CO-STP, -NR-L-CO-TFP, -NR-L-CO-benzotriazole, -NR-L-CHO, -NR-L-maleimide or NR-L-NH-CO-CH2I, where R is H or an aliphatic or heteroaliphatic group L is a divalent linear, crossed or cyclic alkyl group optionally substituted by at least one oxygen or sulfur atom and Kat is a number of Na+, K+, Ca2+, ammonia or other cation(s) needed to compensate for the negative charge brought by the cyanine may be useful as labels for conjugation to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds are provided.
