Mycobacterium tuberculosisMycobacterium tuberculosisThe present invention relates to an antibacterial composition for combating carbapenem resistant gram negative bacteria comprising adenylate kinase (ADK) protein which is derived from as an active ingredient. The ADK protein derived from according to the present invention has an outstanding antibacterial activity against carbapenem resistant gram negative bacteria and can beneficially be used in various fields as an antibacterial composition.