The present invention relates to a beekeeping loading box structure comprising: a beekeeping loading box having multiple outer frames and multiple inner frames erected on both sides of a bottom plate, having a multitier shelf installed between the outer frame and the inner frame, having a workspace formed between the inner frames on both sides, having heat-insulating wall bodies formed on outer front/rear/left/right surfaces comprising the outer frames, on the upper portion of the bottom plate, and on the lower portion of the ceiling, and having an entry/exit door formed through the heat-insulating wall body on one side; multiple honeybee entrances/exits formed through the heat-insulating wall bodies; a cooling/heating device installed on one side of the beekeeping loading box; multiple pairs of guide frames provided between the inner frames; a worktable having a worktable pulley installed to protrude in a position corresponding to that of the guide frames inside the workspace; multiple fixed pulleys and a mo
