Disclosed is an isolated plasminogen variant or plasmin variant obtained therefrom, or an isolated plasmin variant, wherein said variants are comprising an activation site and a catalytic domain, wherein said catalytic domain contains a mutation of the amino acid at position 1 of the human plasmin catalytic domain or at a position corresponding thereto in a non-human plasmin catalytic domain, wherein (i) the amino acid at position -1 relative to the plasmin catalytic domain is an arginine, lysine or other amino acid that maintains functionality of the activation site, (ii) the amino acid at position 24 of the human plasmin catalytic domain, or at the corresponding position of a non-human plasmin catalytic domain, is a methionine, and (iii) the amino acid at position 1 is mutated into an amino acid different from glycine or proline, and wherein said human plasmin catalytic domain is starting with the amino acid valine at position 1 which is the same valine amino acid occurring at position 562 of human Glu-plasminogen.
