LUAN, YEN-TUNG,WANG, WENGE,NYBERG, GREGG,GOMES, JOSE MANUEL,DRAPEAU, DENIS,CARDOZA, TERRY
申请号:
CA2720980
公开号:
CA2720980C
申请日:
2009.04.16
申请国别(地区):
CA
年份:
2014
代理人:
摘要:
Methods and processes for improved recombinant protein production areprovided. The methods are useful forproduction of growth factors, particularly those of the TGF-.beta.superfamily, including bone morphogenetic proteins (BMPs), suchas BMP-2. Suitable host cells are cultured in media where iron is present at aconcentration of at least 2.25 µM and if pyridoxal ispresent, it makes up less than 55 % of the molar concentration of vitamin B6in the media.