PROBLEM TO BE SOLVED: To provide a preservation solution and a method of preservation that exert a superior life-extending effect in non-frozen cryopreservation of bovine embryo.SOLUTION: The bovine embryo preservation solution contains: either a peptide consisting of an amino-acid sequence shown in SEQ ID NO 2 or a peptide consisting of an amino-acid sequence with one or more amino acids therein deleted, substituted, inserted or added in the amino-acid sequence shown in SEQ ID NO 2 and having a cell life prolongation activity, serum and medium. The method for bovine embryo preservation using the solution is also provided.COPYRIGHT: (C)2012,JPO&INPIT【課題】牛胚の非凍結低温保存時に優れた延命効果を発揮する保存液及び保存方法を提供する。【解決手段】配列番号2に示されるアミノ酸配列から成るペプチド又は配列番号2に示されるアミノ酸配列において1若しくは数個のアミノ酸が欠失、置換、挿入若しくは付加されたアミノ酸配列から成り、且つ細胞延命活性を有するペプチド、血清及び培地を含有する牛胚保存液、並びに該保存液を用いる牛胚の保存方法。【選択図】図1