A kind of device and method can be used for separating biological fluid and from the liquid portion for wherein retrieving separation. The device can have a pipe, an insertion piece and positioning mechanism. The pipe can have proximal end, the proximally and distally chamber between distal end, for receiving biological liquid sample. Insert can be located at interior, and can have first end, second end, and between the first and the second ends and draw tube second end is in fluid communication and well. Positioning mechanism may be coupled to pipe and insertion piece, and can be manually actuable, to be collected in insertion piece, proximally and distally to allow the biological fluid of separate section to flow to nearside, in well by draw tube and from the room described.