Methods of treating mammalian subjects suffering from, or believed to be suffering from, acute ischemic brain stroke, cardiovascular disease, inflammatory disease or any of the conditions or symptoms related to those diseases, are disclosed in which a biological fluid withdrawn from the subject is processed through an ozone delivery system to deliver a measured amount of ozone to the biological fluid to produce a treated fluid that has a quantifiable absorbed-dose of ozone which, upon re- introduction of the treated fluid containing the quantifiable absorbed-dose of ozone to the subject, effectively treats any number of described disease conditions and symptoms. Methods for manufacturing medicaments for treatment of acute ischemic brain stroke, cardiovascular disease, inflammatory disease or any of the conditions or symptoms related to those diseases, are also disclosed.
