The objective of the present invention is to provide a novel DNA aptamer useful for the diagnosis, treatment, and prevention of metastasis, and the like, of lung cancer.A DNA aptamer comprising specifically binding to a non-small cell lung cancer cell, in which the DNA aptamer has at least one nucleotide sequence selected from the sequence of SEQ ID NOs: 1 and 2; a composition for detecting lung cancer cell, comprising the DNA aptamer; a kit for detecting lung cancer cell, comprising the DNA aptamer; a method for detecting lung cancer comprising using the DNA aptamer; a detection method comprising contacting the DNA aptamer with a sample obtained from the living body selected from the group consisting of lung cells, lung tissues, blood, serum, plasma, saliva, and sputum and detecting the presence of a lung cancer cell by observing the response caused by the binding of the sample and the DNA aptamer; a pharmaceutical composition for preventing metastasis of or treating lung cancer, comprising the DNA aptamer, and a drug delivery system for preventing metastasis of or treating lung cancer, comprising the DNA aptamer.