A pen type device for ultrasound guided fine needle aspiration cytology and biopsy comprises a hollow body having a needle hole, a sliding part slidably mounted inside the body, elastic members mounted inside the body, a syringe having a cylinder mounted, a rotating part disposed inside the sliding part and the body, a head screw-coupled to the rear end of the sliding part and drawn out from the body, and a negative pressure generating part mounted on the body to generate negative pressure to the syringe by pulling a piston of the syringe when a button located on the outer face of the body is pushed.