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METHODS FOR MAKING NEURAL STEM CELLS AND USES THEREOF
专利权人:
The University of North Carolina at Chapel Hill
发明人:
Shawn D. HINGTGEN,Raluca DUMITRU,Julio RODRIGUEZ BAGO
申请号:
US15554785
公开号:
US20180051249A1
申请日:
2016.03.03
申请国别(地区):
US
年份:
2018
代理人:
摘要:
Provided herein according to some embodiments is a method for producing an induced neural stem cell (iNSC), which method may include one or more of the steps of: providing a somatic cell; introducing into (e.g., by transfecting or transducing) said somatic cell with a nucleic acid encoding Sox2, whereby said cell expresses Sox2; and then transdifferentiating said somatic cell (e.g., by growing the cell in a neural progenitor medium), to thereby make said induced neural stem cell. In some embodiments, the transdifferentiating is carried out for a time of from 1 to 10 days, from 1 to 5 days, from 1 to 3 days, or from 12 to 24 or 48 hours.
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