The present invention relates to a method for producing a silk fibroin (or silk protein) porous three-dimensional support for tissue and bone regeneration through salt leaching using salt and sucrose. Tissue engineering aims at restoration of human function by grafting an appropriate support for the reconstruction of deficient organs and tissues in vivo or by transplanting tissue cells in vitro and transplanting an artificial tissue having a structure and function similar to actual organs or tissues. Therefore, in order to utilize the biomaterial as a support for tissue engineering, a porous structure must be formed. This can provide the space needed for cell growth if the biomaterial itself has adequate pores, and if the pores are interconnected to form an open channel, the intercellular nutrients, metabolites and gas exchange will be smooth. In order to maintain and restore tissue successfully, it is important to control the rate at which biomaterials are degraded, as well as cell adhesion, proliferation, and extracellular matrix formation. Silk fibroin is a natural high molecular weight protein which is sericin-free from silkworm cocoons and can be mass-produced. Its safety has been proven by FDA. Unlike other synthetic polymers, it does not cause immune reaction in the body and has a low inflammation reaction and excellent biocompatibility. It is a medical material that biochemistry and medical application are active because it can control the decomposition rate. Methods for producing a three-dimensional porous support using such silk fibroin include casting, electrospinning, and salt leaching. The salt leaching method has been actively studied. However, there is a limitation in that the mechanical strength is weak for preparing the support using the aqueous solution of silk fibroin, and the preparation time is long after freeze drying process after salt removal. In the present invention, the above-mentioned disadvantages are supplemented to enhance the mechanic
