Sensors for measuring an analyte in a subject comprising at least one electrode having an electroactive surface, at least one interferent-reducing layer comprising vinyl ester monomeric units disposed distally from the electroactive surface, an enzyme layer disposed distally from the electroactive surface and a flux-limiting membrane disposed over the at least one interferent-reducing layer and the enzyme layer. Methods of measuring an analyte in a subject comprising providing the electrochemical analyte sensor, contacting a sample comprising an analyte and an interferent with the at least one interferent-reducing layer comprising vinyl ester monomeric units, and measuring the analyte, where the amount of interferent reaching the electroactive surface is reduced.
