The United States of America; as represented by the Secretary; Department of Health and Human Serv
发明人:
Vince Pozsgay
申请号：
US14318425
公开号：
US20140341909A1
申请日:
2014.06.27
申请国别(地区):
US
年份:
2014
代理人:
摘要：
A method is provided for covalently linking carbohydrates, proteins, nucleic acids, and other biomolecules under neutral conditions, using a Diels-Alder cycloaddition reaction. In an example, activated carbon-carbon double bonds were attached to free amino sites of a carrier protein, and a conjugated diene was attached to a carbohydrate hapten. Spontaneous coupling of the carbohydrate and the protein components under very mild conditions provided glycoconjugates containing up to 37 carbohydrate hapten units per carrier protein molecule. The method is also applicable to the immobilization of biomolecules on gel or solid supports. The conjugated products are useful as immunogens and as analytical and diagnostic reagents.
