FIRST GAIN INTERNATIONAL LIMITED;丰安国际有限公司;豐安國際有限公司
发明人:
WONG, Bing Lou,黄炳鏐,黃炳鏐,KWOK, Sui Yi,郭瑞仪,郭瑞儀
申请号：
MOJ002063
公开号：
MOJ002063B
申请日:
2016.02.26
申请国别(地区):
MO
年份:
2016
代理人:
摘要：
The present invention provides the tetrameric hemoglobins of (optionally ≥70% β-β crosslinking) of a kind of high-temperature stable and highly purified crosslinking, it is suitble to what mammal with efficient oxygen conveying function, the tetrameric hemoglobin without leading to injury of kidney and vessel retraction. The hemoglobin of dimeric forms is denaturalized and the red blood cell from whole blood is purified. Controllable hypotonic lysis is carried out in rapid cellular cracking apparatus to prevent the cracking of leucocyte. The nucleic acid and phosphatide impurity for carrying out leukocytes are not detected in lysate. Pass through mercapto reagent closing activity sulfydryl in oxygen cyclization border. Circulation column chromatography removes different plasma protein impurity. N-acetylcystein is added to the tetrameric hemoglobin of crosslinking to keep low-level ferrihemoglobin. Stable hemoglobin is stored in the infusion bag with aluminium outer packing and invades to form inactive ferrihemoglobin to prevent block. The product what tissue oxygenation and treatment of cancer.本發明提供了一種高溫穩定的和高度純化的交聯的(任選地≥70％β-β交聯)的四聚體血紅蛋白，其具有高效的氧輸送功能，所述四聚體血紅蛋白適合用於哺乳動物而不導致腎損傷和血管收縮。將二聚體形式的血紅蛋白變性並對來自全血的紅細胞進行純化。在快速細胞裂解設備中進行可控低滲裂解以防止白細胞的裂解。在裂解液中沒有檢測到來自白細胞的核酸和磷脂雜質。在氧合環境中通過巰基試劑封閉活性巰基。流通柱色譜去除不同的血漿蛋白雜質。將N-乙酰半胱氨酸加入至交聯的四聚體血紅蛋白以保持低水平的高鐵血紅蛋白。穩定的血紅蛋白保存在帶有鋁外包裝的輸液袋中以防止氧侵入形成無活性的高鐵血紅蛋白。該産品用於組織氧合和癌症治療。
